I have tried to estimate SOD from blood hemolysate by Pyragallol autooxidation method of Marland n Markland.
I have followed the following steps :
BLANK: 50mM Tris EDTA Buffer. (ph-8.5)
Control: TE Buffer + Dist.water+ Pyragallol
Sample: TE + hemolysate + PG.
Observed for 5 mins at 420nm for 5 mins.
But I am getting higher sample Absorbance above the control. I tried diluting till 100x n 500x. But the colour of the hemolysate Is not allowing the proper estimation of SOD percentage inhibition.
I think I am making an error with the grouping. Can anyone suggest me a way to overcome the background influence of the hemolysate using this same method.