How to get rid of background colour of hemolysate in SOD estimation by Pyrogallol autooxidation method??

I have tried to estimate SOD from blood hemolysate by Pyragallol autooxidation method of Marland n Markland. I have followed the following steps : BLANK: 50mM Tris EDTA Buffer....

03 April 2018 8,140 1 View

For comparing effect of a drug on fat percentage on adipose tissues in HFD rats, which is the adipose tissue that should be considered?

I hv HFD treated rats n after treatment with my formulation, I want to compare the effect on the adipose tissue. I want to know which adipose tissue whether BAT or WAT should be considered. And in...

03 April 2018 5,073 1 View

Estimating the Concentration of Acetyl COA & Malonyl COA in Cytosol?

I am trying to study the effect of my drug on the concentration of Acetyl COA and Malonyl COA in Cytosol. Can anyone suggest me a non kit based Colourimetric/Spectrometric study to determine their...

11 December 2017 5,205 2 View

Please mention the technical specializations for MRI and ECG in rats?

Friends please provide me the technical specifications required for carrying out rat MRI and ECG machines and secondly whether normal ECG/MRI machines could be modified or not to suit this purpose...

05 June 2016 5,017 1 View

Suggest me a Software for H & E stained pics ?

03 April 2016 913 10 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View