Quantity One is okay, you can have a try.

Seconding the ImageJ suggestion.

I intend to observe hepatic steatosis and fat diposition in placebo with the treatment groups.

Perhaps you can try Image J, plugin Color histogram.

Thanks a lot everyone for sharing such valuable information.

imageJ is the most oftenly used  free software.

Image J Cell Counter

Image tool or J. Both have counter cell.

You can use Image J. Either automated or manually depending on how accurate you need your data to be

Image J is a good software tool to use plus, if you've not used it before, there is an excellent tutorial on You Tube from the Bio Imaging department at The University of Manchester, UK.

https://www.youtube.com/watch?v=_zgvo6GBdX8