Suggest me a Software for H & E stained pics ?

More Anmol Banerjee's questions See All

How to get rid of background colour of hemolysate in SOD estimation by Pyrogallol autooxidation method??

I have tried to estimate SOD from blood hemolysate by Pyragallol autooxidation method of Marland n Markland. I have followed the following steps : BLANK: 50mM Tris EDTA Buffer....

03 April 2018 8,140 1 View

For comparing effect of a drug on fat percentage on adipose tissues in HFD rats, which is the adipose tissue that should be considered?

I hv HFD treated rats n after treatment with my formulation, I want to compare the effect on the adipose tissue. I want to know which adipose tissue whether BAT or WAT should be considered. And in...

03 April 2018 5,073 1 View

Appropriate gloves n masks for Paroxon assay?

Can anyone please guide me to select appropriate gloves and mask as protection while working with Paroxon.

11 December 2017 3,637 0 View

Estimating the Concentration of Acetyl COA & Malonyl COA in Cytosol?

I am trying to study the effect of my drug on the concentration of Acetyl COA and Malonyl COA in Cytosol. Can anyone suggest me a non kit based Colourimetric/Spectrometric study to determine their...

11 December 2017 5,205 2 View

Please mention the technical specializations for MRI and ECG in rats?

Friends please provide me the technical specifications required for carrying out rat MRI and ECG machines and secondly whether normal ECG/MRI machines could be modified or not to suit this purpose...

05 June 2016 5,017 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Riqiang Bao

Quantity One is okay, you can have a try.

Silvan R Urfer

Seconding the ImageJ suggestion.

Anmol Banerjee

I intend to observe hepatic steatosis and fat diposition in placebo with the treatment groups.

Francisco Tortosa

Perhaps you can try Image J, plugin Color histogram.

Thanks a lot everyone for sharing such valuable information.

Abdelraheim Attaai

imageJ is the most oftenly used free software.

Dmitry Zinovkin

Image J Cell Counter

Hermes Pretel

Image tool or J. Both have counter cell.

Moses Ekong

You can use Image J. Either automated or manually depending on how accurate you need your data to be

Sarah Rushton

Image J is a good software tool to use plus, if you've not used it before, there is an excellent tutorial on You Tube from the Bio Imaging department at The University of Manchester, UK.

https://www.youtube.com/watch?v=_zgvo6GBdX8