How to get fluorescence spectra ?

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How to plot colour map in origin 9 if I have x and y data sheet?

I have one x column and for that x I have 100 y columns as y1, y2, y3 and so on. How to plot colour map in origin?

22 February 2020 9,861 3 View

Among Oxygen and mixture of Oxygen and Argon by 1:1 , which is better for removing CVD grown graphene from Cu foil?

I have Cu foil both side covered with CVD grown graphene. I like to remove graphene from one side without affecting the other side. Now I am using 1:1 ratio of Oxygen and Argon. Is this condition...

03 February 2020 3,689 2 View

How to calculate cumulative drug release ?

I have attached the method as well as calculations for drug release but could not find appropriate results even after several repetitions. It was clearly visible that there must be a high...

11 October 2019 699 3 View

In Sigma Aldrich and Alfa Aesar different type of Nafion solution are available. Which can be used for thin film deposition on FTO?

I have some powder samples. For photoelectrochemical measurement (PEC) measurements, I want to prepare thin film of my samples on FTO. Can any one please help me which Nafion solution I will use...

22 July 2019 1,918 6 View

Can anyone say why hydroxypropylbeta cyclodextrin swells more in basic pH than i n acidic?

I have studied the swelling behaviour and drug release pattern from hydroxypropyl beta cyclodextrin where I have found that the swelling and release % was more in basic compared to acidic. It...

04 July 2019 315 2 View

Can anyone help me analyse the XRD pattern attached?

I have tried to change the structure from CUR to CP. I know XRD is not mandatory here but since I did I would like to analyze the result. A very simple explanation will also be highly appreciated....

26 April 2019 5,459 7 View

Why we use modified stern-volmer equation?

The modified stern-volmer equation involves the assumption that free and total drug concentration are equivalent. Recently, a slightly modified equation to overcome this simplification has been...

08 September 2018 1,884 0 View

Where one can get this modified stern-volmer equation?

log(F0-F/F)= log Kb + n log[D]t The above equation involves the statement that free and total drug concentrations are equivalent. To overcome this simplifying assumption and to get a more...

08 September 2018 7,605 0 View

How to fit a non-linear equation?

Hi everyone, [D]t={(F0-F/F)*(1/Kb)}^(1/n)+n*[P]t*(F0-F/F) where [D]t and [P]t gives total drug conc and total protein conc. we want to determine Kb and n. we have a series of data for y=(F0-F/F),...

08 September 2018 793 4 View

How to fit a non-linear equation?

Hi everyone, [D]t={(F0-F/F)*(1/Kb)}^(1/n)+n*[P]t*(F0-F/F) where [D]t and [P]t gives total drug conc and total protein conc. we want to determine Kb and n. we have a series of data for y=(F0-F/F),...

07 September 2018 873 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View