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Questions related from Samima Khatun
log(F0-F/F)= log Kb + n log[D]t The above equation involves the statement that free and total drug concentrations are equivalent. To overcome this simplifying assumption and to get a more...
09 September 2018 7,720 0 View
The modified stern-volmer equation involves the assumption that free and total drug concentration are equivalent. Recently, a slightly modified equation to overcome this simplification has been...
09 September 2018 1,990 0 View
Hi everyone, [D]t={(F0-F/F)*(1/Kb)}^(1/n)+n*[P]t*(F0-F/F) where [D]t and [P]t gives total drug conc and total protein conc. we want to determine Kb and n. we have a series of data for y=(F0-F/F),...
09 September 2018 870 4 View
Hi everyone We generally use CD and FTIR techniques to see the secondary structure change of protein in presence of drug. Docking we perform to find out binding site of that particular drug in...
07 July 2018 6,546 2 View
We have used FRET method to see the donor(protein)- acceptor(drug) distance which we get for our system as r=1.6 nm. Docking we perform to find out binding site of that particular drug in protein...
07 July 2018 3,632 2 View
We plot modified Stern-Volmer eqn to get 'n' value. For 1:1 stoichiometry case, we do not get the exact value of n=1 rather slight less or more than 1. what is actual reason of this deviation?
11 November 2017 6,974 0 View
Hi everyone If the thermal unfolding temperature of HSA protein after adding drug decreases what does it mean ? is it secondary or tertiary structure which destabilized by drug ? is it suggest...
08 August 2017 2,434 0 View
The fluorescence result (eg: binding constant) at two different excitation wavelength 295 and 280 nm should be same or may be different like at exc wavelength 280 nm Kb is in the order of...
06 June 2017 7,597 5 View
in the well known formula of inner filter effect the Aexe and Aem are the absorbance value of only drug or both protein and drug solution ?
05 May 2017 2,007 3 View
Dear all I have performed ITC for protein and ligand solution.generally we substract heat of dilution of ligand from overall heat for ligand titration into HSA .is it necessary to substract the...
05 May 2017 9,059 5 View
Hellow everyone, i m working on a drug interact with HSA protein.the reported binging const is 10^5 and the quenching is enogh good.but when i did fluorescence with the same drug the quenching is...
05 May 2017 4,722 4 View
Dear all is it compulsory that the thermodynamic parameters we got from fluorescence technique at different temperature must be same as we got from ITC technique? for example we get dH and dS...
05 May 2017 4,723 3 View
i have following question for inner filter effect 1.what is the exact formula for correction of Fobs in inner filter effect? 2. Aexc and Aemi are the absorbance of only drug solution or mixture of...
02 February 2017 3,684 0 View
Fluorescence result of a HSA-drug system proved that hydrophobic result is the main force .In docking both hydrogen and hydrophobic force are there.but ITC result for the same system give...
01 January 2017 3,479 5 View
when one do CV experiment for HSA/BSA it give peak in presence of drug.as it is a electrochemical process,electron responsible for redox process.for biological molecule what is responsible for...
08 August 2016 9,327 5 View
J-value needed for r value from FRET graph ...is it calculate from formula given in the literature ? or any software use for this ? plz answer me if anyone knows it
05 May 2016 2,758 2 View
In ANS experiment by Fluorescence method they use Scatchard plot to calculate Kd(approximate) and Fmax and Surface hydrophobicity index ...what are the steps to plot the graph and to do...
05 May 2016 2,481 0 View
Hello everyone, i am new learner of DFT theory...my work is on protein -drug chemistry...i want to work on DFT ..so from where should i start ?(sp. for basic theoritical portion)...and lastly...
04 April 2016 8,023 5 View
