How to generate PSF and perform Deconvolution?

05 February 2020 1 6K Report

I have recently started learning about deblurring images using deconvolution. I have a stack of images (in .tif format) of size 873 MB (1392 x 1040) and a total of 301 images in a single .tif stack which I want to deconvolve. I do not have the PSF but I have the following details:

Numerical Aperture: 0.3

Refractive Index: 1.00

Emission Wavelength: 620 nm

Specimen Thickness: 3

I need to generate a PSF and then deconvolve the image. I have tried PSF Generator but I don't know why it just stops showing me the image even after running it. I have also tried deconvolutionLab2 but it gets stuck probably because the image is too big.

Please tell me what I can do for an efficient way of deconvolving the image and also generating the PSF.

Olivier Beltramo-Martin

Hello Rounak Vyas

In the case you have diffraction-limited observations, you can estimate the PSF from the aperture shape but a clearer explanation of the optical setup would be needed. Another option could be to explore myopic or marginal deconvolution algorithm if you have priors on your PSF.

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