Storing tissue in Agarose for 24 hrs ?

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My pollutant include dyes. GCMS sample should be devoid of water, i am not getting how to take it in methanol solvent after degradation?

My pollutant include dyes and drugs. GCMS sample should be devoid of water, i am not getting how to take it in methanol solvent after degradation product complete. I have taken the sample and...

01 November 2020 5,840 3 View

What are the basis for determining assessment factors during chemical risk analysis?

I have EC50 and LC50 values and wanted to calculate PEC for different species in water. I have currently considered acute risk with an assessment factor of 100 based on a previous similar...

12 July 2020 4,562 2 View

Is Hemispheric Brain Dominance a current research topic in neuroscience/psychology ?

I want to start research regarding Brain dominance and some concepts of positive psychology. That is why I ask whether or not it is a research area that is relevant now. If not could you please...

09 March 2020 6,857 5 View

How to generate PSF and perform Deconvolution?

I have recently started learning about deblurring images using deconvolution. I have a stack of images (in .tif format) of size 873 MB (1392 x 1040) and a total of 301 images in a single .tif...

04 February 2020 5,866 1 View

What is the Difference beetween crystallite size calculated by PXRD and crystal size observed by SEM analysis ?

let us consider some metal oxide particles, which shows cubic shape with some specific size of a singe cube in SEM image. now if we calculate crystallite size of the same with its PXRD data, then...

09 October 2019 6,326 5 View

How to differentiate T. elliptica, T. tomentosa, T. crenulata and T. alata? Different or synonymous?

Going through every literature and floral publication, they have their own theories, some say different species some consider synonymous to each other. There is a need of Key to Terminalia species...

08 September 2019 3,029 3 View

Does an elastomer expand the same way every time it's stretched?

Say there is an elastomer strip of length L, width W and uniform thickness -T. All other parameters like material density, temperature are constant. I mark this strip with N points starting...

25 October 2018 740 3 View

Can we leverage CIM (Common Information Model) framework for using big data in power industry?

Can we leverage CIM (Common Information Model) framework that is already in place through IEC standards (IEC 61970, 62325) for the big data use cases in utility industry??

13 September 2018 4,762 1 View

Can a Bioanalyzer be used to test for DNA fragmentation?

I am working on a project using DNA from fairly old tissue specimens (>30 years). We will be extracting DNA from these samples for sequencing, but we want to see how fragmented the DNA extracts...

31 January 2018 3,636 1 View

Negative slope in the curve of iron concentration and T2 relaxation?

I've done MRI imaging of iron oxide nanoparticles in phantom gels. The images are showing decreased signal intensities upon increasing the iron concentration, this is due to iron oxide being a...

15 December 2017 3,379 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View