Dear Colleagues,

After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on PVDF.

Since PVDF has been blocked with skim milk, does colloidal gold bind to the blocking proteins on PVDF?

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Also, I would like to have the most efficient colloidal gold staining protocol that can be used on PVDF after ECL.

I want to get opinions about my concern above.

Thank you

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