I am working on a project using DNA from fairly old tissue specimens (>30 years). We will be extracting DNA from these samples for sequencing, but we want to see how fragmented the DNA extracts are.
We want to use an Agilent Bioanalyzer to do this. Does anyone here know of a protocol or have one they can recommend?
Yes you can use a bioanalyzer to look at DNA fragmentation. The bioanalyzer will give you a distribution of sizes of DNA relative to the ladder provided and the quality of the DNA. Aligent provides lovely protocols of how to use the DNA chips on the bioanalyzer, these can be found at : https://www.genomics.agilent.com/en/Bioanalyzer-DNA-RNA-Kits/DNA-Analysis-Kits/?cid=AG-PT-105&tabId=AG-PR-1040.
Also there are different kits to use depending on the amount of DNA you are measuring and you only require 1ul of you material. I would recommend using this to check your DNA, you defiantly will be able to see if the samples are of poor quality.
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