According to the Clontech manual for yeast one hybrid kit, it recommends generating a mutant bait sequence that disrupts binding of targeted TF(s). Since I'm targeting mainly bZIP family TFs based on multiple online tools (PlantRegMap, PLACE, PlantCARe, Transfac, etc), I've shortlisted a 25bp region in my target promoter that I'd like to use as bait. Could anyone let me know how would one synthesize the mutant bait fragment?
For eg., if my target oligo (+) reads: TCCTCACGTGGGTCCTCACGTGGG and MEME predicted the core motif as TCACG, what would the mutant bait sequence be?