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Using Matchmaker Yeast one-hybrid screening system, I have obtained around 1.8-2 million clones and carrying forward from here, I performed colony PCR as per recommendation. However, most of the...
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According to the Clontech manual for yeast one hybrid kit, it recommends generating a mutant bait sequence that disrupts binding of targeted TF(s). Since I'm targeting mainly bZIP family TFs based...
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