How to gate CD4, CD8 and NK cells from PBMC?

04 January 2019 1 6K Report

I used to gate CD4 and CD8 from CD3+ cells. In my recent experiment i wanna gate CD4, CD8 and NK cells from PBMC. can someone recommend me the gating path for NK cell?

Thanks.

Shen-An Hwang

There are probably multiple answers to this... but you can use the same strategy since NKs should stay in the lymphocyte cluster.

They would be CD3- and CD161+ (I use 161 as the NK marker). If they are CD3+CD161+ than I classify them as NKTs.

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