How to calculate multiplicity of infection(MOI)?

04 March 2020 4,208 3 View

How can we stain liposomes with rhodamine for fluorescent microscopy?

I want to stain liposome with rhodamine. For this I need protocol.

20 April 2019 7,138 1 View

What should be the sonication time to disperse Magnetite nanoparticles for SEM?

I am using probe ultrasonicator to disperse MNPs in ethanol for SEM.

25 January 2019 2,091 8 View

How to calculate efficiency of the working electrode without material deposition?

how we can use Potassium hexacyanoferrate to check efficiency of working electrode?

24 June 2018 6,773 3 View

How to check activation of electrode with Potassium ferricyanide solution?

what is the procedure to prepare the Potassium ferricyanide solution.

19 June 2018 2,567 4 View

Can anyone give me suggestions regarding peaceful personality? i.e. how to measure it? any instrument?

can anyone give me suggestions regarding peaceful personality? i.e. how to measure it? any instrument?

20 August 2015 3,817 7 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to apply predefined temperature to generate prestressed force in Abaqus?

Dear researcher, I'm applying a thermal strain to generate the prestressed force in the steel strand of my concrete wall model using predefined temperature in Abaqus as shown in Figure 1. In...

26 January 2021 7,058 7 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Is it possible for peeling off pedot:pss film on bare glass?

I try to fabricate free stranding pedot:pss film. I already prepare pedot:pss film by using OTS treated glass. But in many papers, they just using bare glass not OTS treated glass. I did it, but I...

05 January 2021 6,968 1 View

Loss of reporter gene expression from lentiviral transfer plasmid?

We have produced a 3rd generation, self-inactivating lentiviral vector for the expression of our gene of interest under the EIF1alpha promoter. This vector also contains the GFP reporter gene...

22 December 2020 4,918 3 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

Is the expression of House keeping genes disturbed in an abnormal situation like in a diseased tissue?

In gene expression studies, what would be the reliability of house keeping genes expression say in a disease/insect attacked tissue that is being subjected to studies (RT qPCR ). Will the...

14 December 2020 3,445 5 View