If we design proteins like tetrabody or triabody with unique binding sites in silico without further wet lab validation can we patent that structure and procedure?
This is something that should be discussed with a patent attorney or agent in the specific jurisdiction which you aim to submit the patent. An article that covers the particulars of protein sequences and patents can be found in link [1].
Also, even if the sequence is not patentable, its process of "construction" may be and the particular tools could also be patentable, if they are unique, novel and non obvious.