Hello - planning a SYBR Green qPCR study. I have primer efficiencies, including for reference genes, from 90-105%
I am planning to use efficiency correction in my calculations - do I correct for efficiencies > 100% ? Or do I assume a 100% efficiency for those primer pairs (I know this may not be the case) ?
There is plenty of information on efficiency calculation or estimation but not a lot about what to do with these efficiencies, what are the acceptable ranges for ref gene efficiencies etc.
Many thanks for you contribution