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Questions related from Saleha Arooj
Hi All: I need to precipitate extracellular protein from supernatant. A bit confused with ammonium sulfate calculation. Should I be adding: 1. 65g of ammonium sulfate in 100 ml of...
10 July 2024 5,464 0 View
So, I am working on a small protein (X) of Haloferax volcanii. It shows these results: 1. Mutant strain grows poorlycompared to wild type strain under low iron condition. 2. Growth of Mutant...
15 May 2024 8,910 4 View
Hi everyone I want to introduce some iron stress to haloferax cells in the culture. Normally, i have been applying low iron stress where i just prepare media with low iron (Hv.Min minus 23 mg...
29 January 2024 5,247 0 View
Hello everyone I used WebFlaG for flanking genes homology search but I am not able to understand the result output completely. How to know what are these flanking genes? (digits for example). Does...
16 January 2024 8,093 0 View
Hi everyone Its a small protein with 5.7kD weight. After doing weight adjustments considering acidic amino acids and also 3XFLAG tag, it should be 11.6 kD. But on 16% tricine gel, it appears...
15 August 2023 5,525 7 View
Hello all researchers: I have started working on a hypothetical protein. In silico (SWISS-prot, Hhpred, I-TASSER) analysis shows its homology to following proteins: 1. Hydrolase (peptidase) 2....
27 April 2023 5,983 2 View
Hello scientists: I have a small archaeal gene (168bp), its on the reverse strand. How can i find the promoter? How to be sure about the promoter sequence? Thanks.
09 February 2023 5,890 0 View
I need some help with the calculation: Probe lenght: 500bp required 10nM ug/ul=? Considering ave.molecular weight per base= 660g/mol I am either getting very high amount or extremely low, using...
14 November 2022 4,451 2 View
I have been trying different concentrations (1259ng, 2250ng) in hybridization solution (50ml) but no signal. (I tried with control also where there was just the probe against probe). Or is it...
08 November 2022 6,368 1 View
I was going to perform Northern Blot. After running the samples on agarose gel, one of the samples did not seem to travel. Albeit performed northern blot, no signal at all for all the samples....
26 October 2022 3,301 3 View
I need to extract RNA at OD(650nm) 0.1-0.15. With this OD, harvested pellet is too too small. I am following Nucleozol protocol. Earlier, for 0.5-0.7 ODs, i was using 20ml and was getting decent...
23 September 2022 1,476 0 View
Hi all: I am working on a small (60a.a) archaeal protein. Scenario is: Deletion strain shows better growth under low Fe conditions than the wild type strain. At the same time more siderophores...
01 January 1970 5,276 3 View
