09 October 2017 2 4K Report

Can you please suggest me any method to achieve this?

More Gozde Kabay's questions See All
Western blot result of Transfected cells , Can you please explain and make suggestions for troubleshooting about my western blot?

Hello, My protein of interest in 104kDa. I transfected Hek293T cells with plasmid and then run western blot. The last two lane is transfected cell lysate. But as you can see I cant see a thick...

07 March 2023 5,233 0 View

How do we prepare 2.5 uM BSA in a DMEM-LG media? What should be stock concentration?

Hello all, I need urgent help. I have to prepare incomplete media whic includes '2.5 uM BSA' in it. How should i prepare stock ? As I am going to use BSA in incomplete media can i thaw the BSA...

06 February 2023 564 1 View

What is 0.2% 'aqueous' formic acid means and how do you prepare it ?

Hello everyone, I would like to do UPLC. In the protocol there is 0.2% aqueous formic acid and 0.2% formic acid in methanol. what is aqueous means and how do we prepare them? Thank you for your help

08 December 2022 8,684 5 View

How to calculate % change from placebo (% intergroup difference between study and control groups) of a marker measured in a clinical trial?

Hi everyone, I have been trying to figure out a reliable way regarding how to present % intergroup difference of a marker level between study and control groups in a clinical trial and I am...

01 August 2022 6,121 2 View

How to do advance search on PubMed for analysing side effects of a drug type (statins to be more specific)?

Hello everyone, Currently I am working on writing a review of side effects of statins and will do further analysis regarding which statin type has the best side effect profile. However, I am new...

13 May 2021 8,354 6 View

What is the charge density of PDDA (Polydiallyldimethylammonium chloride)?

Dear members, For my coating experiments, I tried to measure the surface charge/charge density of PDDA with a conductometric titration, however, I couldn't reach a proper result.I wonder ıf...

22 December 2020 7,557 1 View

How can I achieve gold nanoparticle modification by amino groups?

How can I functionalize Au np suspension with amino groups? I also want to drop N-Au nps onto carbon electrode surface. Is there anyone to help me?

20 July 2018 4,778 2 View

How come calcein positive cells are also PI positive?

I am working with NIH 3T3 cells at the moment and I want to do live/dead assay. I use Calcein-AM and Propidium Iodide. I have done several trials so far. However, no matter what I do I keep...

12 May 2015 6,329 9 View

Would anyone like to work together to adapt the Behavioral Regulation in Exercise Questionnaire-3 into multiple languages?

I plan to adapt Behavioral Regulation in Exercise Questionnaire-3 in Turkish. Let s work cross-cultural!

07 April 2015 1,424 18 View

What type of medium should I use for culturing human aortic smooth muscle cells (HASMCs) and human aortic endothelial cells (HAECs)?

Hello everyone, We will start culturing HASMCs and HAECs soon in our lab and I am trying to get ready. However, I am confused regarding what type of medium to use. I have done a literature scan...

09 March 2015 6,411 11 View

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