Make a calibration plot for phenol and 2,4-DCP. Take the absorbance of the pollutant sample and calculate max abs for phenol and DCP, followed by make your calculation

Use a mixture of phenol and 2,4-DCP as blind sample. In the other cuvette, place the standard pollutant that you prepared earlier and added to the mixture of phenol and 2,4-DCP, of certain concentrations and increasing. A graph will be created. When you give the sample, the device will convert the absorbance to the concentration. The sample concentration should not be too low or too high for standard solutions.

Dear Praveendouga, your analytical question is not as easy as it seems. The major problem is that both spectra (phenol and 2,4-DCP) are relatively well overlapped. Certainly, you have separate standards for both. Record separate UV spectra for both and compare. As absorbance is additive, the spectra of a mixture will be the result of a sum of the individual spectra, which will be a function of the concentration of each compound in the sample. So, I strongly suggest that you use a multivariate calibration, in which you will prepare a set of standards containing samples with only one analyte but also with mixtures between them, in different levels of concentration. You will record all those spectra (say, between 200 and 400 nm) and use these data to build a calibration model, whose absorbance in a given wavelength will be a function of the concentration of each analyte and also a function of the absorbance in other wavelengths. Then, you will record the UV spectra of your sample and input the required absorbances that you calibration required into your calibration function and solve the equation system for the unknown concentrations. You can read the following article to help you a little bit more: Article Spectrophotometric determination of phenol in the presence o...

Thank you so much Leshan Usgodaarachchi , Mehmet Can Dal and Edmar Martendal . I agree with Edmar Martendal