many hydrolysis process to convert the glycone to aglycone flavonoids to be easy to extract in plant crude . which one is the best, fast ,simple and safe
thank you very much
Acidic hydrolysis is used when glycosides of phenolic compounds should be cleaved and only the aglycons are analyzed. Often this is also of interested. Especially when there is a lack of authentic standards (e.g. for exotic flavonoid glycosides). However, acidic hydrolysis can be very harsh and might lead also to a slight degradation of the aglycon backbone. So in some cases it can be of interest to use an enzymatic hydrolysis procedure.
No, there is no real procedure. When you use HPLC chromatography you can follow the peaks diappearing (the glycosidic structures) and the strong formation/increase of single peaks (the aglycons). To see if the acidic hydrolysis is to harsh, there can be also the formation of further peaks representing smal degradation products (when hydrolyzing flavonoid glycosides often protocatechuic acid formation is an indicator).
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