Please researchers, I need brain MRI datasets comprises dyslexia and control urgently?

Dear Researchers, I need brain MRI datasets that comprise of dyslexia and control. The data could be inform of images or binary format or any other standard format. You can link me up to websites...

18 November 2019 7,806 3 View

Dear colleagues, are the problems contained in the attached document researchable in Deep Learning and CNN? What are the current issues in Deep model?

Dear Colleagues, I'm currently on my PhD research in Deep Learning for Brain Image Analysis. Kindly help me check the attached Problem Statement and advise if those problem researchable. If they...

13 October 2019 355 1 View

Need Wood and Bright 1992 Catalog of bark beetles?

Need pdf version of Catalog--- Wood, S. L. & D. E. Bright (1992): A catalog of Scolytidae and Platypodidae (Coleoptera), Part 2: Taxonomic index. - Great Basin Naturalist Memoirs 13: 1-155....

27 April 2019 6,532 1 View

Quantitative of total phenol content wave length 650 nm?

24 August 2017 9,417 3 View

Which prefer solvents ratio(mixture) for polyphenol extraction?

i want to extract polyphenol from a certain plant in both hot and cold alcoholic extraction: what is the suitable mixture ratio of solvents for both hot(soxhelt )and cold alcoholic...

15 May 2017 6,718 3 View

How to Compute/construct of eigenfunctions of perturbed nanoparticle from unperturbed eigenfunctions?

How do I obtain /compute the eigenfunctions (wavefunctions) of unperturbed nanoparticle with inner radius (a),outer radius(b) of a complete spherical particle with a thickness (b-a=t) loaded with...

20 March 2017 3,594 12 View

How to use non parabolic band approximation for the analysis of interband effects of electrons for plasmonic device application

How to use non parabolic band approximation for the analysis of interband effects of electrons for plasmonic device application?

23 October 2016 9,526 1 View

Which model will produce best results at modelling message routing in a computer network? neuro-fuzzy system or genetic algorithms?

Which model will produce best results at modelling message routing in a computer network? neuro-fuzzy system or genetic algorithms? Also, is it possible to hybridize the two models to enhance...

23 August 2015 5,524 3 View

I am working on climate change impact on a primate species endemic to Niger delta. How do i know the impact of climate change on the species?

i need to know the method i can use to determine the effect of climate change on the species distribution and abundance

01 January 1970 5,401 7 View

Can I use phase field to model Reinforced Concrete Beams with Abaqus program?

Can I use phase field to model Reinforced Concrete Beams with Abaqus program?

01 January 1970 6,542 1 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

No title

The tested drug is a chemotherapeutic agent the type of the cell lines is colorectal cancer cell lines the cell viability was compared to the control my thought was the cells were activated by...

02 February 2021 1,256 11 View

Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View

Can we use Spray dried solid lipid nanoparticles for MTT assay?

I have synthesized Quercetin SLNs and spray dried them using Mannitol to avoid drug leakage. However, I need to perform MTT (cytotoxicity). Can I use the powder directly disperse it into media and...

08 January 2021 6,783 3 View

Which substance can I use as positive standard in MTT assay on A431 cell line (human epidermoid carcinoma)?

Hi everyone, I am planning to do an MTT assay for the cytotoxicity evaluation of several plant extracts on A431 cell line (human epidermoid carcinoma). For this I need to use a positive standard....

14 December 2020 1,470 4 View

The reason of gene silencing?

We integrated a gene in a cell line genome, when we culture them, the gene turn to silencing. We collected non-silencing cells and culture them, some of them turn to silencing again. We already...

02 December 2020 3,529 2 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Do anyone know a NK cell line expressing FC receptor to perform ADCC assay?

Hello All, I wanted to do Antibody-dependent cell cytotoxicity (ADCC) assay. Has anybody tried it using NK cell line that express Fc receptors such as CD64, CD32. Can you please share what cell...

16 November 2020 4,472 3 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View