How to extract eDNA from biofilm forming bacteria and how to evaluate the presence of eDNA in agarose gel?

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Beatriz Panariello

Dear Subhaswaraj Pattnaik,

I'm not sure if this will help you, but I extracted eDNA from Candida albicans biofilms and the protocol I applied can also be used for bacterial biofilms. However, I didn't evaluate eDNA in agarose gel.

Mert Sudagidan

Dear Subhaswaraj, in the following papers some protocols related to eDNA was described.

Best regards.

The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus

http://www.pnas.org/content/pnas/104/19/8113.full.pdf

Contribution of the Staphylococcus aureus Atl AM and GL Murein Hydrolase Activities in Cell Division, Autolysis, and Biofilm Formation

http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0042244&type=printable

Paweł Krzyżek

This article includes the methodology of extraction of eDNA from bacterial biofilm and detection of eDNA by agarose gel electrophoresis. It applies to H. pylori, but it may be useful.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679919/

Subhaswaraj Pattnaik

Thank you all for sharing important information. I will try to do the work as per the given protocol and if any queries are there I will get back to you.