How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to see the expression of 6kda protein?

Dear all, I am now investigating the expression of small size protein (6 kda). However, the expression of the protein cannot be shown clearly after performing western blot. Therefore, are there...

13 January 2021 3,121 3 View

Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

16 December 2020 5,795 3 View

Why in BET surface area becomes zero but when detag ads des, it shows good value around 150-200? For single crystals is it a good data to publish?

For single crystals can I give BET data in the publication if it is coming around 200. And why it shows zero value surface area but after detag ads or des it shows value, and which one I should...

02 December 2020 7,173 1 View

How to analyse tga curve, when weight loss is not matching with constituents present in structure?

In TGA curve I am getting 2 step decomposition in which the 1st one coming around 185 to 195 degree centigrade. The weight loss calculated is not matching with the constituent present. Its a metal...

20 November 2020 714 5 View

Colony PCR, why my colony PCR result is negative, but the colony can grow in 100 ug/ml amp LB medium?

Hi all, I have picked the colony with pipette and tip the colony directly with the PCR mix. The forward primer and reverse primer that I checked is ok with the melting temp 60 C. Then I...

21 January 2020 6,086 9 View

Urgent! Please help. Problem about cloning and mini-prep. Why I get low concentration of DNA after mini-prep?

In the last few weeks, I have successfully clone a plasmid that consists of GFP and pUC19. Both shows clone on the plates and no clone in negative control plate. After overnight culture with...

17 December 2019 2,352 10 View

How do you obtain all consensus exon sequences from WES?

After bwa assembly to the reference genome I end up with sorted and indexed BAM files, but I am interested in understanding how to properly obtain all exon consensus sequences (to produce...

08 October 2019 9,970 2 View

How much is N concentrations in the fern?

08 February 2017 1,284 3 View

Can you share your Experience related to new Limnological methods especially related to Sampling the Planktons and their Preservation?

09 October 2016 1,739 2 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

Should I use Pressure Based Solver or a Density-Based Solver?

Dear researchers, I want to simulate a flow (air) in a wind tunnel, and I want to integrate a fluidic actuator inside it in order to control the separation of the boundary layer. At the blower...

22 February 2021 4,882 3 View

Help with assigning pressure boundary conditions in FLUENT on a large structure?

Hello, I created a very tall tower (>1000m) and applied pressure boundary conditions at the inlet (bottom) and outlet (top) that correspond to the pressure are their relative altitudes....

22 February 2021 9,972 4 View

How can I obtain Reaction Forces in ABAQUS?

I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...

21 February 2021 3,075 2 View

How to implement boundary conditions in Abaqus contact problems?

Hello I am trying to simulate crawling motion of a bilayer beam using Abaqus. Kind of similar to this: https://youtu.be/os-Z3ucoFpA I am using multiphysics enabled (diffusion, deformation,...

20 February 2021 2,916 1 View

Abaqus: deactivate displacement boundary condition?

I am running analysis of a cross-shaped sample that is prescribed a displacement along x and y-axes. I have run several jobs with this model and had no issues. However, the current set of...

15 February 2021 1,680 8 View

I have some problems with the mass transfer part of the CFD simulation of membrane distillation.Can anyone help me?

I am working on modeling of Direct Contact Membrane Distillation system with COMSOL Multiphysics software. I have some problems with the mass transfer part of the simulation because I don't know...

14 February 2021 379 2 View