Need suggestion, good ideas and research articles
Hybrid energy systems are systems that combine two or more sources of energy, such as solar and wind power, to generate electricity. Research on hybrid energy systems aims to improve the...
27 January 2023 5,058 4 View
There are several ways to obtain solar irradiance data for a specific location in India. Some of the most common sources of this data include: National Renewable Energy Laboratory (NREL) - NREL...
27 January 2023 1,838 6 View
Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...
15 March 2020 6,939 4 View
We have isolated several Quercetin glycosides form some plant sources. We need Hyperin for the comparison purpose. In case of your help will be gratefully acknowledged.
13 September 2018 5,484 2 View
Hello friends, I am working on synthesis of sugar based silver nanoparticles. I confirmed its presence by the yellow colour formation. It was formed by mixing of 10 mL of 1 mM silver nitrate with...
06 November 2017 6,956 7 View
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03 April 2017 5,512 1 View
What are the common primer's (F/R) used to detect bacteriophage endolysins (lysine) and tail holin?
11 November 2015 4,064 1 View
Dear phage experts, I recently tried TEM analysis for my isolated phage (H. W. Ackermann, 2011 protocol), but I could not get images and results. Can anyone help me to analyze my phage by...
26 January 2015 9,981 17 View
Dear phage experts, I am very much interested to work with phage RNA in the absences of commercial kit. anybody Help/ Guide me for extraction of bacteriophages RNA and give me suggestion/ share...
07 January 2015 1,205 0 View
I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.
24 December 2014 6,612 6 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
