Extracting Stiffness Matrix From LS-Dyna?

Hello, I am actually trying to optimize an armour plate for now. For this purpose, I need the stiffness matrix of the plate. While I can write a Matlab code to compute this it will be hard to...

02 November 2020 7,213 3 View

How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

15 March 2020 6,690 4 View

How can I compare compressive strength from crushing concrete cube specimens to strength of in-field reinforced concrete structures?

1. In general, a lot of literature is available on compressive strength of cube or cylinder specimens. However, in practice, reinforced concrete is used in the field, especially for large...

29 October 2019 1,665 11 View

With amperometric Ab biosensors.How do we ensure that the Potential change is not due to interaction of other biomolecules with electrode?

In case of an amperometric antibody based biosensor where we use graphene as an electrode is it possible that additional impedance is created due to other biomolecules that have the capacity to...

14 February 2018 142 2 View

Please can any one suggest me how to connect two seperateley optimized molecular fragments into one in gaussian 09?

16 July 2017 1,677 5 View

How to simulation of thin film nanocomposite by COMSOL for gas adsorption?

simulation of thin film

05 April 2017 6,298 1 View

What are the latest methodologies used in diagnosing cervical cancer?

26 April 2016 3,759 3 View

Could anyone help me with TEM analysis of my bacteriophages?

26 January 2015 9,759 17 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

24 December 2014 6,470 6 View

How do we index the ZnS and CdS Quantum dot crystal planes spacing from high magnification HRTEM images?

Crystal lattice planes are observable in HRTEM images and we can find the lattice plane spacing. My confusion is how we index the crystal planes as (100) ,(111) etc. How we make sure observed...

19 September 2014 6,321 2 View

Can we use Spray dried solid lipid nanoparticles for MTT assay?

I have synthesized Quercetin SLNs and spray dried them using Mannitol to avoid drug leakage. However, I need to perform MTT (cytotoxicity). Can I use the powder directly disperse it into media and...

08 January 2021 6,783 3 View

Can U suggest standard protocols of quantifying total phenolics & total flavonoids using gallic or tannic acid for the former and quercetin for later?

The problem is that everytime getting the flavonoids content much higher than phenolics which may be, but in rare occasion. In phenolics, I am adding100 ul sample with 900 ul dH2O with...

29 September 2020 4,993 6 View

What is the best protocol of western blot for protein detection and using PVDF membrane?

i am planing to look at alpha glycosides but I I have PVDF membrane and I will use semi-dry method but how I should do western blot and how prepare blocking buffer after running the membrane in...

27 August 2020 6,304 3 View

Calculation of TOtal phenolic content and total flavonoid content?

19 July 2020 761 5 View

How to find Quercetin conent in solvent?How to test Quercetin that is formed?

My project is extraction of quercetin from onion peel.How to find quercetin concentration in solvent?Extraction is done using soxhlet extractor.Solvent we used is Ethanol.Done with different...

09 March 2020 945 3 View

Chromaflex Standard Chromatography setup?

I am attempting to recreate an extraction of steroidal glycosides from Lily bulbs from this paper Isolation and Structural Determination of Steroidal Glycosid... To do so I need to run GPC on a...

28 January 2020 9,047 2 View

What is the best procedure to isolate the Quercetin and Resveratrol from their sources?

BEST EXTRACTION AND ISOLATION PURIFICATION METHOD FOR QUERCETIN AND RESVERATROL FROM PLANT SOURCES? (i.e)Quercetin from Apple and Resveratrol from Blueberries? AND also does phenol exist in...

08 December 2019 9,884 5 View

My drugs have higher CAA values than quercetin. Is this logical or did I do something wrong?

Hello, I performed CAA assay with N-acetylcysteine (both free and nano-formulated drugs), quercetin on SHSY-5Y cell line. However, CAA values for those drugs are higher then the quercetin. Any...

07 August 2019 5,475 1 View

How can I remove chlorophyll from leaf extract without losing other secondary metabolites?

I am working on isolation, purification and characterization of Quercetine from leaf extracts. I have acetone extract of leaf and I want to remove the chlorophyll from it first. i tried...

07 August 2019 4,929 7 View

How to calculate log p of quercetin?

I am taking equal amount of octanol and water and adding sufficient quantity of drug in the mixture. But log p is coming 3.66 whereas reported value is 1.52

24 March 2019 2,542 2 View