Nattan Stalin

12 Questions 20 Answers 0 Followers

Questions related from Nattan Stalin

How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

16 March 2020 6,833 4 View

What are the common primer's (F/R) used to detect bacteriophage endolysins (lysine) and tail holin?

12 November 2015 4,021 1 View

Could anyone help me with TEM analysis of my bacteriophages?

Dear phage experts, I recently tried TEM analysis for my isolated phage (H. W. Ackermann, 2011 protocol), but I could not get images and results. Can anyone help me to analyze my phage by...

27 January 2015 9,851 17 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

25 December 2014 6,554 6 View

Why did I get a pellicle formation during the inhibition of Vibrio alginolyticus biofilm using specific phage?

Any help would be greatly appreciated.

13 August 2014 5,124 3 View

Why do my Vibrio species change their nature of yellow color to green color on TCBS plates after 36 hour incubation?

I have more than 10 Vibrio species in my lab. When I started purification (streaking) on TCBS plates, they were a yellow color within 24 hour incubation at 35˚C, when I continued their incubation...

23 May 2014 6,844 8 View

How can I prepare bacteriophage sample for transmission electron microscopy?

I followed given methodology: Collected phage buffer containing phage sample 10ml , centrifuged at 12000 rpm for 10 min, top supernatant 10µl collected and diluted 10-5 of phage sample 100 µl...

21 May 2014 8,802 6 View

Why did I get a small amount of plasmid DNA during bacterial Genomics DNA extraction/ isolation?

see above

10 March 2014 2,431 6 View

How to extract and purify the Bacteriophage particles against pathogenic bacteria? - please suggest me good ideas and research articles

Need suggestion, good ideas and research articles

18 June 2013 7,064 0 View

Could anyone tell me how to calculate the adsorption rate of bacteria and bacteriophage? with any time duration and/or mathematical calculation

13 June 2013 1,085 3 View

How can I stop bacterial growth after titration/stored SM Buffer?

...

07 May 2013 4,740 5 View

How to prepare adherence cell line from THP-1 suspension cell line ?

Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?

01 January 1970 9,954 1 View