Nattan Stalin

18 Questions 30 Answers 0 Followers

Questions related from Nattan Stalin

How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

16 March 2020 6,861 4 View

Could anybody share their protocol for Mycobacterium bacteriophage isolation from aquatic environment?

04 April 2017 5,480 1 View

What are the common primer's (F/R) used to detect bacteriophage endolysins (lysine) and tail holin?

12 November 2015 4,032 1 View

Could anyone help me with TEM analysis of my bacteriophages?

Dear phage experts, I recently tried TEM analysis for my isolated phage (H. W. Ackermann, 2011 protocol), but I could not get images and results. Can anyone help me to analyze my phage by...

27 January 2015 9,917 17 View

How to isolates bacteriophage RNA ?

Dear phage experts, I am very much interested to work with phage RNA in the absences of commercial kit. anybody Help/ Guide me for extraction of bacteriophages RNA and give me suggestion/ share...

08 January 2015 1,174 0 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

25 December 2014 6,577 6 View

Why did I get a pellicle formation during the inhibition of Vibrio alginolyticus biofilm using specific phage?

Any help would be greatly appreciated.

13 August 2014 5,134 3 View

Why do my Vibrio species change their nature of yellow color to green color on TCBS plates after 36 hour incubation?

I have more than 10 Vibrio species in my lab. When I started purification (streaking) on TCBS plates, they were a yellow color within 24 hour incubation at 35˚C, when I continued their incubation...

23 May 2014 6,863 8 View

How can I prepare bacteriophage sample for transmission electron microscopy?

I followed given methodology: Collected phage buffer containing phage sample 10ml , centrifuged at 12000 rpm for 10 min, top supernatant 10µl collected and diluted 10-5 of phage sample 100 µl...

21 May 2014 8,809 6 View

How could I maintain/store bacteriophages for a long time?

I am unable to store marine bacteriophages for a long time with an SM buffer at -4 C°, -20 C° and -80 C°. Please share the correct protocol for this.

22 March 2014 3,589 4 View

Why did I get a small amount of plasmid DNA during bacterial Genomics DNA extraction/ isolation?

see above

10 March 2014 2,455 6 View

Does anyone know, whether this double layer phage plate is good or not?

06 January 2014 8,164 7 View

How to extract and purify the Bacteriophage particles against pathogenic bacteria? - please suggest me good ideas and research articles

Need suggestion, good ideas and research articles

18 June 2013 7,090 0 View

Could anyone tell me how to calculate the adsorption rate of bacteria and bacteriophage? with any time duration and/or mathematical calculation

13 June 2013 1,111 3 View

How can I stop bacterial growth after titration/stored SM Buffer?

...

07 May 2013 4,748 5 View

Can anyone suggest the best articles for extraction/ isolation of DNA/ RNA from Bacteriophage without using any kit?

03 May 2013 9,451 4 View

Can anybody please share their protocol for marine bacteriophage isolation ?

I am doing research in marine phage therapy and I have been trying to isolate phage for the last 3 months but I could not find phages from marine samples.

01 December 2012 128 4 View

How to prepare adherence cell line from THP-1 suspension cell line ?

Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?

01 January 1970 9,980 1 View