Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Can you correct the wt%/at% of an incorrectly assigned peak in EDS?

For instance, I get a spectrum and the software incorrectly assigns a Si peak as W. If I don't catch it on the software and only have the report (which lists both wt% and at%), is there a way to...

10 February 2021 7,665 4 View

What's wrong with my sample stained with MAP2 antibody?

I've studied and fabricated a nanostructured substrate for the enhancement of neuronal differentiation. I usually confirmed the levels of differentiation using immunocytochemistry, but I recently...

20 September 2020 5,725 3 View

How can I calculate or see bond angles in Gaussian16?

In Gaussian program, I calculated formaldehyde using "opt+frq". In the results, I can see the bond length and so on, except bond angles. How can I see the bond angles in that program? Thank you...

30 July 2020 7,165 3 View

Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

Control sample: cells that have not been treated with drugs, only DMSO in a MTT assay displays detachment from plate, why would that be?

The control sample set of the MTT assay is shown to have detached from the plate as seen by the clear area (without the formazan crystals), when even cells that were treated with low levels of...

19 March 2020 4,670 3 View

Does color or lighting/reflection influence behavioral test in mice?

Our pole test apparatus includes a metal pole covered with white cotton thread, with a grey plastic base that gives a slight reflection. In our experiment, many mice reach within a few centimeters...

17 January 2020 9,969 1 View

How can I analyze height of nano-pattern sample through scanning electron microscopy (SEM)?

Hello, I'm fabricating nano-pattern through LIL(laser interference lithography). I want to check the height of pattern sample through SEM, since our lab has it. I've tried to check the height of...

01 July 2019 2,733 3 View

Is it possible to take AFM measurement of Graphene oxide sheet with Iron oxide particles?

I'm wondering it's possible to take AFM measurement of Graphene oxide sheet conjugated with micro-sized Fe3O4 particles. If possible, which mode (e.g. contact, non-contact, and tapping) is the...

06 June 2017 5,577 4 View

Real-time fluorescence imaging for insulin secretion?

22 March 2017 7,480 2 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View