Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Can you correct the wt%/at% of an incorrectly assigned peak in EDS?

For instance, I get a spectrum and the software incorrectly assigns a Si peak as W. If I don't catch it on the software and only have the report (which lists both wt% and at%), is there a way to...

10 February 2021 7,665 4 View

What's wrong with my sample stained with MAP2 antibody?

I've studied and fabricated a nanostructured substrate for the enhancement of neuronal differentiation. I usually confirmed the levels of differentiation using immunocytochemistry, but I recently...

20 September 2020 5,725 3 View

How can I calculate or see bond angles in Gaussian16?

In Gaussian program, I calculated formaldehyde using "opt+frq". In the results, I can see the bond length and so on, except bond angles. How can I see the bond angles in that program? Thank you...

30 July 2020 7,165 3 View

Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

Control sample: cells that have not been treated with drugs, only DMSO in a MTT assay displays detachment from plate, why would that be?

The control sample set of the MTT assay is shown to have detached from the plate as seen by the clear area (without the formazan crystals), when even cells that were treated with low levels of...

19 March 2020 4,670 3 View

Does color or lighting/reflection influence behavioral test in mice?

Our pole test apparatus includes a metal pole covered with white cotton thread, with a grey plastic base that gives a slight reflection. In our experiment, many mice reach within a few centimeters...

17 January 2020 9,969 1 View

How can I analyze height of nano-pattern sample through scanning electron microscopy (SEM)?

Hello, I'm fabricating nano-pattern through LIL(laser interference lithography). I want to check the height of pattern sample through SEM, since our lab has it. I've tried to check the height of...

01 July 2019 2,733 3 View

Is it possible to take AFM measurement of Graphene oxide sheet with Iron oxide particles?

I'm wondering it's possible to take AFM measurement of Graphene oxide sheet conjugated with micro-sized Fe3O4 particles. If possible, which mode (e.g. contact, non-contact, and tapping) is the...

06 June 2017 5,577 4 View

Real-time fluorescence imaging for insulin secretion?

22 March 2017 7,480 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View