Methods for non-destructively calculating hedges biomass?

Hello, I intend to calculate the biomass and carbon of intact hedges looking at above and below ground components. Could someone please recommend an accurate and verified technique for recording...

06 December 2020 9,681 2 View

Troubles cloning - correct RE digestion and then incorrect sequencing?

I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...

23 November 2020 7,962 2 View

How to extract gene expression data from the Phylofish database for a specific gene?

I am having trouble navigating the phylofish database. I can blast my gene of interest and get the " Best match," but from there, I cant seem to figure out where to go to find the expression level...

15 September 2020 4,882 2 View

How to count % GFP positive cells and GFP intensity using imagej?

I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...

17 August 2020 4,009 2 View

Can a poor performance result be written for a paper?

I am currently working on a database and I have tried various methods of machine learning and deep learning, all leads to poor classification/prediction. However, this might show that the two...

27 May 2020 7,894 7 View

Can I use soft vacuum filtration to harvest Ath seedlings from liquid media?

Hello, I'm growing different accessions of Arabidopsis thaliana and so far we have been harvesting them by pouring the media over a simple sieve, then putting them on paper towels for a minute...

18 February 2020 7,944 3 View

What is the best device for presenting white noise signals to a participant during TMS-EEG?

I'm trying to come up with a method for presenting white noise signals to a participant during TMS-EEG without using a laptop. If you have experience with this what devices do you use? Or should I...

13 February 2020 479 4 View

Western blot sample preparation: What is the best way?

What reagent do you use to dilute your cell lysates after finding protein concentration with Bradford assay? Do you use 1X loading buffer or do you use water? If you use the 1X loading buffer to...

16 January 2020 2,517 3 View

What is the current state of Agent-Based Modeling and Complex Systems research in Computer Science?

I am looking to enter the field of Complex Systems or Complexity Science as a postgraduate in my Computer Science faculty, specifically focusing on Agent-Based Modeling. However, I am unclear on...

04 January 2020 9,084 3 View

What are some good methods papers on morphometric measurement and analysis of herbarium specimens?

I'm looking for a good authoritative source that walks me through methods for morphometrics.

01 August 2018 4,877 8 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View