Currently need to scrunch the polymer off the mandrel, which generates many wrinkles, does anyone have any alternatives?
Hello, I have been attempting to express a protein in Pichia pastoris GS115 for some time now. I have just discovered via wesetrn blot that all of my protein is being precipitated/forms into this...
09 April 2024 9,448 6 View
How can digital technologies improve student learning?
07 August 2023 1,070 7 View
We will make our Agile Authoring development platform available to researchers need to collect data through a sophisticated algorithmic decision tree. The license will be free for researchers....
20 July 2023 2,981 4 View
Does anyone know what microbe we are dealing with in our cell culture lines? We are thinking yeast but are not sure. Vero cells seem very susceptible to it.
04 May 2023 8,097 4 View
Hi all, once again electroporation of Pichia proves troublesome. I am electroporating GS115 strain with a pPIC9K plasmid. Following three days of growth, both the MD and YPD plates contained pink...
12 February 2023 3,131 3 View
I use this chambered culture well plates for immunofluorescent confocal microscopy:...
04 February 2023 4,912 2 View
To keep this question short, let's say I have a novel membrane protein with the known structural fold of a known transporter superfamily so I know its function is a transporter of cellular...
17 January 2023 7,543 2 View
Dear all, I am seeking help in regards to narrowing down my topic for my masters thesis in International Business & Management. My research proposal was recently rejected by my supervisor,...
18 March 2022 8,946 4 View
Hi all, First time working with yeast and I have some questions. Following electroporation (1800V) of my linearized pPIC9K plasmid into GS115 I grew them up on MD agar as per the Invitrogen...
13 February 2022 6,882 2 View
I am in the process of choosing a topic for my thesis and want to investigate organisational structures in MNE's. I am thinking about investigating and comparing the participation between MNEs...
27 December 2021 594 6 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
04 August 2024 1,078 1 View
Details of the Analysis. Static Analysis Composite Layup Continuum Shell Elements FRP Material (Elastic and Hashin Damage)
03 August 2024 8,538 4 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
28 July 2024 9,545 6 View
Sure, here is the translation: I have a 3D orthogonal woven composite structure where warp, weft, and binder yarns are oriented in three directions. After modeling these yarns and the matrix in...
28 July 2024 8,169 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View