I want to check the content of Furfural, acetic acid,HMF in my hydrolysate through Spectrophotometric method or GCMS for which I want the method with reference.
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...
02 July 2024 9,807 3 View
Is it necessary to add stop solution in Elisa plate?
30 June 2024 8,235 2 View
Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...
24 June 2024 1,242 0 View
We are willing to make notice of Miller indices present in XRD powder and managed as their JCPDS files. We need them for various substances, e.g. Graphite, copper, lithium, and Iron. Kindly share...
23 June 2024 864 2 View
After Plagiarism checking in Turnitin, the result shows that the similarity of my manuscript is from ''Article with reviewer- feedback from Springernature'' and the link provided opens a word file...
20 June 2024 8,325 1 View
I have tried to screen various phytochemicals of different plants leaves sample with antimicrobial property. I have tried aqueous extract of plants. But i have doubt whether sample is actually...
18 June 2024 8,947 4 View
research on mental health and ethnicity
18 June 2024 3,958 0 View
It is from 7-hydroxy-4-methyl coumarin with HMTA in dil acetic acid at 90 degrees for 6 hrs and hydrolysed with 20% HCl, kept for 30 min. cooled overnight and extracted with ether.
15 April 2024 3,941 0 View
I am planning to do mass spectrometry for protein hydrolysate. How can I desalt the peptide mixture without affecting hydrophilic and hydrophobic peptides?
25 March 2024 6,663 2 View
How much quantity of sulphuric acid is required for acid hydrolysis of chemically purified cellulose powder to produce crystalline nanocellulose?
03 March 2024 6,264 3 View
The microbe is yeast (Saccharomyces cerevisiae) and acid hydrolysis is the route of treatment. A good paper highlighting all the necessary steps and sequences would be highly appreciated since...
29 February 2024 3,412 2 View
Hi together, does anyone have a detailled protocol for the quantification of ROS in an electrolyte solution, i.e. in the absence of any cells? I am currently working on this with the fluorescent...
07 February 2024 8,690 3 View
Are there any simple colorimetric or other methods that do not need expensive sophisticated equipments?
06 January 2024 2,583 0 View
I am trying to extract lipids using water as solvent. After extraction followed by centrifugation, I did not obtain any oil layer. Instead the supernatant was milky white, which I think might be...
30 October 2023 9,151 1 View
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28 September 2023 7,665 3 View
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27 September 2023 7,197 3 View
27 September 2023 4,452 3 View