Elhadad Nesma

A novel method for the separation of peptides from amino acids and salts has been developed. Separation is achieved by passage of the sample through a column of octadecasilyl-silica

Article Purification of peptides: An efficient procedure for the sep...

To desalt peptide mixtures while preserving their hydrophilic and hydrophobic properties, you can use a reversed-phase solid-phase extraction (SPE) method. This method separates peptides based on their hydrophobicity using a hydrophobic stationary phase, while the mobile phase is a water-miscible organic solvent. The method allows for the elution of hydrophilic peptides first, followed by hydrophobic peptides using a stepwise increase in the organic solvent concentration. Alternatively, size exclusion chromatography (SEC) can also be used to separate peptides based on their size and hydrophilicity. SEC separates peptides by size, with larger peptides eluting first, and smaller peptides eluting later. This method can also be used to desalt peptide mixtures without affecting their hydrophilic and hydrophobic properties.