The microbe is yeast (Saccharomyces cerevisiae) and acid hydrolysis is the route of treatment. A good paper highlighting all the necessary steps and sequences would be highly appreciated since this is the first time I am undertaking such an experiment.
Hello Adarsh Shetty
The following articles might be helpful for you.
Article Autolysis, plasmolysis and enzymatic hydrolysis of baker's y...
Article Flash hydrolysis of yeast (Saccharomyces cerevisiae) for pro...
Thanks
Chandan
I don't think there is one standard protocol for this question. It will be dependent on the strain of S. cerevisiae that you are using and on the nature of the hydrolysate. Is your strain tolerant to inhibitors in the hydrolysate or is it a 'lab' yeast? Are you wanting papers that describe how to prepare the hydrolysate or just papers growing yeast in hydrolysates. Are you just looking for growth or will you have a product other than biomass? If a product, which? Growth conditions will be dependent on requirements for the product - e.g. to produce ethanol you want low oxygen or anaerobic, but to produce something like xylitol you want an aerobic culture. It is often a good idea to do a small-scale preliminary experiment to assess whether the hydrolysate is toxic and if so how toxic. (e.g. a set of low volume flasks with a series of dilutioins of they hydrolysate such as 100, 75, 50, 25% hydrolysate with a constant amount of added sugar, inoculate and assess whether all sugar is consumed in all concentrations). From this you can then define a proper experiment with replicates. If the hydrolysate is very toxic, you may also need to make a 'synthetic' medium in which you add each potential inhibitor in a separate flask so you can assess which ones are most toxic. And of course start with a good analysis of the hydrolysate itself, to know what it contains.
There are numerous reviews on hydrolysates which should provide links to the literature describing how to produce the hydrolysate. There are also reviews on further pre-treatments if they seem necessary. And abundant literature on growing S. cerevisiae in various hydrolysates. I recommend looking not at a single paper, but at several so you have an idea of the range of problems you may encounter. If the strain you are using has been published about before, focus on publications which have used it. If the material that is being hydrolysed is something that has been used before, than focus also on those publications.
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