On aqueous extraction of oil what is the white milky layer that is obtained as supernatant?

31 October 2023 1 9K Report

I am trying to extract lipids using water as solvent. After extraction followed by centrifugation, I did not obtain any oil layer. Instead the supernatant was milky white, which I think might be an emulsion (correct me if I am wrong). Could anybody tell me how do I obtain the oil layer from the milky white supernatant?

#aqueousextraction #lipids #proteinhydrolysate #emulsion

Zoë Gaffen

I would try a low speed centrifugation: the lipids should then coalesce into one layer, above the water, and can be removed by suction.

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