6 Questions 1 Answers 0 Followers
Questions related from Marta Węgierska
Hi! I need to find and probably buy cell line(s) that is/are positive to CD34+ CD43+. I need them as a positive control to both these markers. Do you have any reccommendations? Are there any...
24 February 2023 3,953 1 View
Hello! I am using a protocol from Iriguchi et al. to obtain HPC and then lymphocytes. They want us to coat plates with rhDL4/Fc protein and retronectin. They say: "Dilute rhDL4/Fc chimera...
06 February 2023 5,446 0 View
Hello! It's me and Embryoid Bodies again. When it comes to total protein or mRNA isolation from EBs it's fine. But I have to admit that BCA shows low amount of total protein after lysis... But it...
10 January 2023 3,959 2 View
Hi! A week ago I started a culture of SK-N-F1 cell line with NF1 mutation. We obtained it from ATCC (https://www.atcc.org/products/crl-2142). I am the only person in lab that works with these...
22 November 2022 266 1 View
Hi! Right now I am working with embyioid bodies generated from iPSCs (PBMC) to obtain CD34+ cells. After 2 weekes of culture flow cytometry has to be performed. It is highly problematic because...
27 October 2022 3,933 0 View
Hi! I want to stain embrioid bodies that I have in culture using immunocytochemistry method. I also want EBs not to completely flatten out on the plate - it would be perfect if they'd remain 3D...
20 October 2022 5,906 0 View
