I purchased 200kDa Hyaluronic acid (HAc) powder and want to make a hydrogel for dermatological fillers with it.
In many kinds of literature, it was confirmed that the gelation process was performed using a crosslinking agent called BDDE for the gelation of HAc in NaOH.
With this in mind, the experiment was conducted as follows.
1. Completely dissolve 10% HAc in 0.25N NaOH. (120rpm, 30 minutes to 1 hour)
2. Add 1% BDDE crosslinker to the completely dissolved HAc solution and continue the reaction. (40 ºC, 120 rpm (magnetic bar), over 6 hours)
It is said in the literature that gelation is easily achieved through this process.
But, I do not have gelation.
Is there something wrong with my method?
*As you can see in an uploaded picture, it is in solution even after 24 hours, and it only changes from transparent color to brown, but gelation does not proceed....
* Is it correct to continue mixing with a magnetic bar during the crosslinking process?
Dear Kyeongwoo Lee, please consider the following. Sodium Hyaluronate is to be used and the pH should be maintained at about 12. Please have a look at the following document. My Regards
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197218/#:~:text=As%20it%20has%20a%20few,)%20as%20cross%2Dlinking%20agent.
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