If i want to do my research data analysis what i should start with?

like a guide especially with putting the score for my survey. *my survey will measure knowledge level for the disease population Moreover, then which tool should I use? My study is a...

18 January 2021 3,819 10 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

What is the ideal cell number for detection of HIF using ELISA ?

Hi All, I am planning to measure HIF expression with different cellular conditions using ELISA kit, from your experience what is the ideal number of cells used for this type of experiment?

07 December 2020 3,066 3 View

Why I got different sizes with polyacrylamide nanoparticles when I measure the size by DLS? I've prepared them with same procedure?

I've prepared nanoparticles by inverse microemulsion technique and I've functionalised the nanoparticles with N-(3-aminopropyl)-methacrylamide (APMA) (+ve charge) to facilitate cellular delivery,...

02 December 2020 8,329 3 View

My protein ladder is diffused and take a lot of time for running electrophrosis?

hello, I am doing western blot for cancer cell and my protein ladder diffused during running electrophoresis and the sample is disappear. also it takes too much time more than 3 hr for migration....

14 September 2020 5,113 4 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

How to model VLC V2V communication system in matlab?

I want to simulate what will be BER vs SNR if i sent a stream of bits through VLC in outdoor system; V2X or V2V. any help??

09 February 2020 6,478 4 View

How to remoe imaginary part of Zin in hfss?

i am working on 6 MHz antenna and i want to remove the imaginary part in the input impedance. i used Lumped RLC bounday condition but no sol. any help?

11 September 2018 2,320 3 View

How to import mimics file to ANSYS workbench?

17 August 2016 4,200 4 View

Is it feasible to do cell viability test by counting cells using trypan blue in a 96-well plate? How can I detach cells from wells exactly?

02 January 2016 5,165 6 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View