How to dissolve PVA in chloroform for binder preparation?

23 November 2022 0 6K Report

I successfully dissolved 1% PVA in water for 8 hours. Need to know protocol for Chloroform.

Badges
Science topic

More Ashutosh Dinanath Bagde's questions See All

Hi all how one can calculate C:N:P ratio?

Dear all, Will you please let me know how to calculate the carbon, nitrogen, and phosphorus (C: N:P) ratio in wastewater? Is it advisable to measure total COD, nitrogen, and phosphate? or I...

05 February 2024 4,105 3 View

How can i change bond type in pdb file?

Hi i am doing work on a protein which has Cu ion in it but the bond it is making with protein are not matching the reference protein making bond with Cu ion so is there any possible to change the...

23 January 2024 5,999 1 View

Regarding phyco-remediation cattle derived wastewater?

Hello intellect. I am working with an algal consortium to treat wastewater derived from cattle (CRWW). Different dilutions of raw CFWW (FCWW/dH2O; v/v) were used for algal cultivation. As a...

20 January 2024 341 1 View

Does SPHY 2.1.1 or SPHY 2.0.3 model consider Temperature lapse rate?

I have used WFDEI meterological data from the HinduKush Himalaya database provided by FutureWater to force temperature data on my study area. If the model is not considering temperature lapse...

18 January 2024 7,006 0 View

It is possible to implement real time dairy processing plant effluent remediation using algal consortium?

Being a biotechnologist, I am always curious to know the real example where a stakeholder shows enthusiasm and implements an algal consortium or axenic culture to treat raw dairy wastewater...

30 November 2023 6,761 1 View

Re: Running and Installation of SPHY Model?

I'm trying to learn SPHY model but I'm facing some issues in running of SPHY preprocessor model in QGIS 2.8.2 like GDaL open failed and while creating initial maps they get removed automatically....

16 September 2023 7,614 1 View

How to use data of internal resistance vs DoD and entropy change vs DoD for battery modeling in Ansys Fluent?

The battery models available in Ansys Fluent are NTGK, ECM, and P2D. Most of the research articles have done experimental investigations to find the data of internal resistance vs. DoD and entropy...

14 September 2023 7,171 0 View

Virtual Molecular Dynamics showing blank screen after opening .pdb and .gro file ?

it shows this and vmd shows blank screen Info) VMD for LINUXAMD64, version 1.9.4a57 (April 27, 2022) Info) http://www.ks.uiuc.edu/Research/vmd/ Info) Email questions and bug reports to...

30 August 2023 826 0 View

Molecular docking of small substrate have binded strongly but have lower docking energy ?

can we consider -5 kcal/mol binding energy is as good energy for small molecules like serotonin in molecular docking

23 August 2023 7,146 2 View

Why do I see a diferent crystal structure when uploaded in seek k-path from vesta created crystal. And how do eneter the highsymetry k-points in QE?

12 August 2023 3,571 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

What solvent should I use to make a Schiff base from a ketone starting material?

I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...

23 June 2024 5,925 3 View

Hello.I have a starting material that has both carboxylic acid and alchohalic OH group.I added triphosgene to convert thoes groups to acid cholorid?

and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?

14 June 2024 2,924 3 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

Separation of Cholesteryl esters from alkanes/alkenes?

Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...

02 June 2024 5,545 0 View

How to extract good quality RNA from Brown Algae?

We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...

30 May 2024 356 0 View

How can I salvage a failed trizol/chloroform RNA extraction using the first interphase?

I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...

23 May 2024 2,475 1 View

How to obtain a cleaner RNA solution?

Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the...

21 May 2024 9,910 1 View

How to increase yield and purity of RNA from muscle of EEL using TRIzol??

For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...

15 May 2024 3,024 2 View