Being a biotechnologist, I am always curious to know the real example where a stakeholder shows enthusiasm and implements an algal consortium or axenic culture to treat raw dairy wastewater (RDWW). If you are planning to implement this system, then you may face many barriers, like:
1. temporal change in RDWW
2. unsterile RDWW has its microbiota; maybe it competes with the algal consortium
3. Which cultivation system does it adopt: an open raceway pond or a closed system?
4. the flow rate of incoming RDWW.
6. Which mode of cultivation is more suitable for real-time RDWW treatment (batch or semi-continuous)?
7. After or before, which kind of operation enhances the remediation capability and is ready to use or discharge in natural water bodies?
8. How do you maintain the C/N/P ratio?
9. What about the smell of RDWW due to protein purification?
Would it not be better to use the RDWW as a feedstock and use modified algae to make chemical products? Issue 2 can easily be fixed by UV or thermal treatment. Open raceways would have higher problems with sterility unless antibiotics are added. As amino acids from RDWW would provide a constant source of carbon and nitrogen only phosphorous depletion would be a concern. With point 9 I believe you mean putrefaction which could be resolved by using algae lacking the amino acid catabolism pathways that generate odorous compounds eg. cysteine and arginine.
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