I am looking for methods to distinguish between these two cases. For instance, in literature, the Botryosphaeriaceae fungi have an endophytic stage, on the other hand, some Colletotrichum species have a quiescent infection.
Leonardo Brandão I am afraid that only plant reaction can differentiate endophytic (probably beneficial for host) and pathogenic (quiescent / latent infection, thus harmful) fungi. I remember that somebody estimated that >60% of plant infection is present in latent stage (symptomless) .
Endophytic fungi and fungi with quiescent infections can both colonize plant tissues without causing obvious disease symptoms. However, there are some differences between these two types of fungal infections:
Location: Endophytic fungi colonize the inner tissues of plants, such as leaves, stems, and roots, whereas fungi with quiescent infections remain on the surface of the plant tissues, such as the epidermis or cuticle.
Symptomology: Endophytic fungi generally do not cause visible disease symptoms in plants, whereas fungi with quiescent infections may cause mild symptoms, such as discoloration or small lesions.
Lifestyle: Endophytic fungi typically form a mutualistic relationship with the host plant, providing benefits such as increased growth or disease resistance, whereas fungi with quiescent infections are usually parasitic, taking nutrients from the host plant without providing any benefits.
To differentiate between endophytic fungi and fungi with quiescent infections, various techniques can be used, such as:
Microscopy: Microscopic examination of the infected plant tissues can reveal the location of the fungal colonization, as well as the structure and morphology of the fungal structures.
Molecular methods: DNA sequencing or PCR-based techniques can be used to identify the specific fungal species or strains present in the infected plant tissues.
Histological staining: Staining techniques such as PAS or Lactophenol Cotton Blue can reveal the location and extent of fungal colonization within the plant tissues.
Metabolite analysis: Endophytic fungi often produce specific secondary metabolites that can be detected using chemical analysis techniques, whereas fungi with quiescent infections may not produce these metabolites.