How to determine cells flow direction between tissues using flow cytometry and having 1 time measurement for several samples?

24 February 2017 1 7K Report

I have results of FACS (cells % and absolute numbers) for T cells subpopulations in blood, spleen, lymph nodes and bone marrow. Measured once for several samples at the same time point. Is it possible to determine cells migration direction having only that information? Thanks!

Rafael Franco

Hi Valery

If I understand you well you only have a time point ("measured once ... at the same time point"). If so, it is impossible to determine migration as it requires time.

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