Can I ask did any good platform or journals for learning the calcluation on the reference governors and command governor?

Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...

23 February 2021 7,746 2 View

Does anyone know the pH-differential method?

I use the water method to extract the anthocyanidin from Peristrophe bivalvis (L.) Merr leave, then dilute 20 times with 0.025M potassium chloride, pH1.0 and 0.4M sodium acetate buffer, pH4.5...

20 January 2021 8,305 4 View

Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View

TOP/FOP-FLASH assay in HEK293T cells?

Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...

28 August 2020 6,052 4 View

Does anyone have experience in docking metalloenzymes using dock 6.9?

14 August 2020 976 3 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

15 March 2020 3,293 4 View

Is it any way to do partition of edges not in same plane in Abaqus?

I am developing a model in Abaqus to simulate the cornea. I now want to partition the solid so as to set the middle part and outside part with different materials. However, I am having difficulty...

11 December 2019 7,645 1 View

What GLP-1 receptor antibody is good for IHC?

I'm trying to detect GLP1-R on mouse pancreas, I used proteintech 26196 1 AP at concentration 1:100, but it doesn't seem to work. Is there any other GLP-1R antibody that works well for IHC in...

25 November 2019 4,456 2 View

Where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

30 May 2019 2,711 2 View

How to determine the composition of a complex sample containing organic compounds, amorphous and crystalline compound containing copper?

The sample is an intermediate product in the synthesis of CuO-doped TiO2 catalyst. Ethanoic acid is added and it reacts with copper nitrate to produce copper(II)acetate. A gel is prepared with sol...

15 January 2019 6,796 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Possible problem for showing 0.2 sun intensity instead of 1 sun intensity in AAA class Newport solar simulator?

We have a AAA class Newport solar simulator. We changed the Xenon Arc Lamp recently (XBO450W, Make:OSRAM) as the lifetime got over. However, the intensity is coming to be only 0.2 sun instead of 1...

14 February 2021 9,998 3 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View