Hello everyone! I would like to inquire about the MIC test for detecting drugs against Helicobacter pylori. Now I use the micro-broth dilution method: add the drug and bacterial solution into the 96-well plate, where the final concentration of bacterial solution is 1000000CFU/ml, and shock at 150rpm for 72h under 37℃ and micro-aerobic environment. However, after 72h, it was found that there was no bacterial growth in the pore plate, even in the growth control hole. Have you ever encountered such a problem? How is it usually solved?

Note: I conducted the experiment under normal environment, not under the micro-aerobic operating system. Will this affect the growth of bacteria?

Thank you very much！