It is so hard to detach the cells by trypsin. I am using a scrapper for this. But when I do it I can not get sufficient cell amounts. Is there any
easy way to do it?
The driver seems DNA based on the parallelisms below? (?a nonstop dominance struggle in DNA between progressive and retrogressive forces?) Evidence for the parallelism between extreme warmings...
09 June 2023 6,230 10 View
How can I sort works in Bibliography published by the same author in the same year as 2016a/2016b? Like this: Hendriks, F., Kienhues, D., & Bromme, R. (2016a). Hendriks, F., Kienhues, D., &...
30 October 2022 5,248 0 View
In many publications, chrysin has been studied at different absorbances. Which standard should I take? How is chrysin quantification done by HPLC?
08 September 2022 5,878 0 View
I am trying to isolate bacterial genomic DNA from lactobacillus which is incubated +4 degree (around one week), and in a tube (inside liquid media). But I could not get a high DNA concentration...
16 December 2018 3,958 4 View
I study on Formica rufa and effect on litter decompostion and nutrient release. I used a litter bag medhot and I put a litterbag adjasent of ant nest and 10m distance on the forest...
19 November 2018 2,366 3 View
We are working on needle decomposition (Pinus nigra) and seasonal changes of microarthropods and microbial activity in the semi-arid region
22 September 2017 6,462 4 View
Hi everybody, I am looking for a good antibody to detect GPR120 in mice. There are multiple commercially available AB out there. I have tried the AB NBP1-00858 from NovusBio but I got, despite...
06 June 2017 2,500 2 View
I was studying on decomposition in pure and mixed stands. Single-species litterbags were separately filled only with leaf litter of Quercus or Fagus in pure stands while mixed-species litterbags...
12 February 2017 7,568 7 View
ı will examine the possible effect of -lavandula stoechas extract -main substance of lavandula s. extract. Some literatures says especially 'fenchone', some say 'camhor' or others. ı will examine...
08 April 2015 9,231 7 View
I am researching about different dental identification methods such as manually or automatically? I want to know which automated dental identification method is used lately by DVI team or by...
30 November 2014 8,851 6 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View