What is the best way for quantification of a FlAG-tagged protein?

Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated. Thank you

11 January 2021 8,458 1 View

Could Anyone suggest the protocol for staining CD 4 and CD 8 in whole blood. Which buffer is suitable for the staining?

I am staining the CD4 and CD8 markers in the whole blood sample. However, when I used the PBS buffer (pH. 7.0 to 7.4) I have no results. Then I tried 2mmEDTA PBS buffer but still, there is no...

24 March 2020 252 3 View

Where can I buy vaccinia immunoglobulin (VIG)?

Hi, I need to perform a neutralization assay for an orthopoxvirus. I thought VIG should be easily available to purchase. However, I could not find a company to purchase it from. Most people seem...

04 February 2020 6,369 2 View

How to proceed for the counting of CD4, CD8 and CD19 from whole blood sample without isolating the WBC?

I am doing experiment with BD antibody conjugated with CD4 FITC, CD8 PE and CD19 perCp. However, I am not able to detect with higher concentration also. I need some information regarding the...

23 October 2017 8,042 2 View

How to count the live and dead cell and how to distinguish except the trypan blue technique. As, I know trypan blue only stains the dead cell?

05 February 2017 2,591 3 View

What solvent (and concentration) is suitable to dissolve essential oil (water or DMSO)? Is there any complication for using it for cell test?

30 September 2015 9,883 13 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View