What is the best way for quantification of a FlAG-tagged protein?

Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated. Thank you

11 January 2021 8,458 1 View

Where can I buy vaccinia immunoglobulin (VIG)?

Hi, I need to perform a neutralization assay for an orthopoxvirus. I thought VIG should be easily available to purchase. However, I could not find a company to purchase it from. Most people seem...

04 February 2020 6,369 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How can I calculate volume fraction of MWCNT from a nano hybrid composite ?

I am adding 0.1wt percent MWCNT to glass fiber epoxy composite and want to determine the volume fraction of the MWCNT from this composite but I don't know how to calculate it. can anyone help me...

09 February 2021 8,883 3 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Is there any protein soluble in oil?

Hello, I am looking if proteins or protein fractions soluble in oil exist? For food application Thank you for your help

01 February 2021 2,103 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

How to solubilize protein?

Hello, I have cloned my gene of interest in a bacterial expression system i.e. BL 21 Gold (DE3) and checked for protein production and solubility. Good amount of protein was observed on SDS-PAGE...

15 January 2021 7,063 5 View

What is the best way to simulate the fractional controllers?

What software do you prefer?

29 December 2020 889 3 View

How to clean a packed glass column with C18?

I loaded a polar fraction on a glass column packed with C18 and washed it isocratically with 10% methanol for 6 days. The color of this fraction remained on top of the column, then I washed it...

28 December 2020 7,884 3 View