Hello Arvindkumar Hiralal Chaurasiya

I am attaching a research article on the above mentioned subject. I hope it will be helpful.

Article Asymmetric transport of a fluorescent analogue by human erythrocytes

Best Wishes.

Quick search - https://pubmed.ncbi.nlm.nih.gov/4039191/

I have never done it myself, but I wonder if Hb autofluorescence may impact the assay. I understand this may not be feasible in your lab, but we use 13C-glucose and mass spectrometry readouts - Article Oxidative modifications of glyceraldehyde 3-phosphate dehydr...

Hi Arvindkumar,

I've assessed 2-NDBG uptake in the past in my laboratory using T cells with flow cytometry. It is pretty much the same principle with erythrocytes. You can refer to the paper suggested by Malcolm (that I've attached to this message). But to answer your question, YES you can read erythrocytes by FACS.

If you want to be sure that there isn't a autofluorescence of Hb by flow cytometer, you could read unstained cells and see if theres a difference with stained cells.

Dont forget to use media without glucose so you have accurate results.

Goodluck with your experiment!