Ribonuclease H (RNase H) degrades the RNA strand in RNA-DNA hybrids. However, some Reverse Transcriptase has been genetically altered to remove the associated RNase H activity. For example, Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H–]), is an RNA-dependent DNA polymerase that can be used in cDNA synthesis, which lacks RNase H activity. Accordingly, if Reverse Transcriptase, RNase H Minus is used in RT-PCR, How to degrade the RNA strand of RNA–DNA hybrids in the absence of RNase h domain?
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