Hi everyone,
I am currently trying to screen the behavioral effects of the 6-OHDA unilateral injection in the striatum of Wistar male rats to confirm a Parkinson's animal model by apomorphine-induced turning behavior test (or rotameter test).
Although the dose of apomorphine is prepared according to the articles and is injected intraperitoneally to the rats, but instead of turning against the injection site of the neurotoxin, the rats become extremely relaxed and even fall asleep, and it is not possible for me to check the behavior.
I have request from dear students, professors and scientists that help me to find out what the main problem is and share the tips of rotameter test with me.
Please feel free to have contact with me.
Thank you for reading and helping!
Initially I assumed that your 6-OHDA injection may be too shallow, as the dorsal striatum mediates wakefulness, but that still didn't make any sense. Then I dug up this gem from 1979 (it has an English version):
Article Sommeil induit par petites doses d'apomorphine chez les rats
Apparently low doses (like 100 ug/kg SC) of apomorphine makes rats sleepy. So my guess is either your dosing is off or the injections aren't reaching the intraperitoneal space properly. Like maybe they're going into the bowel or bladder or something, although I can't imagine how that could be a regular thing.
Hope this helps!
Hi Shayesteh Kokabi Hamidpour
Please find the detailed protocol attached.
RefArticle Time course of striatal changes induced by 6-hydroxydopamine...
Thanks
Samir
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