Hello,
Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically, slides are producing drying artifact and bubbles after being coverslip for about 1 hour to 24 hours. I have increased mounting media, change clearing reagents more often, agitate the racks while staining and yet this issue keeps lingering. This is causing frustrations with pathologists and causing delay in patient care diagnosis. Any help that can resolve this issue will be much appreciated. Thank you.