How to culture KG1a (nonadherence) cells? KG1a cells seems to be highly sensitive cells and not easily amplified. Does anyone have recommendations for the best culture medium?
You should maintain cultures between 5x105 to 106 cells/ml. Do not culture below 105 cells/ml; 5% CO2; 37°C. IDMEM cell culture medium is better than RPMI1640. It is better to use 2mM Glutamine + 20% Foetal Bovine Serum (FBS) for cell culture.